Hotspot

rps8-rpl14

LSC · 79862-80138 · 277 bp

Back to Glycine

Pi 0.0295

MarkerSeek score 66.80

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 277
variable_sites: 19
pi: 0.0295
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	TTATGTCAGCGATGGTGTCC	GTTTCATTAGCACCCGAGGT	50.0	50.0	57.7	57.9	324-352 bp	1.0	83.2
rps8-rpl14_p2	GTCAGCGATGGTGTCCTTAC	GTTTCATTAGCACCCGAGGT	55.0	50.0	58.4	57.9	320-348 bp	1.0	82.9
rps8-rpl14_p3	TATGTCAGCGATGGTGTCCT	GTTTCATTAGCACCCGAGGT	50.0	50.0	58.5	57.9	323-351 bp	1.0	81.6
rps8-rpl14_p4	TTATGTCAGCGATGGTGTCC	AGTTTCATTAGCACCCGAGG	50.0	50.0	57.7	57.6	325-353 bp	1.0	80.9
rps8-rpl14_p5	TTATGTCAGCGATGGTGTCC	AATCTTTTGTGCAATCGCCC	50.0	45.0	57.7	57.6	376-404 bp	1.0	80.5