Hotspot

atpI-atpH

LSC · 47089-48178 · 1090 bp

Back to Glycine

Pi 0.0179

MarkerSeek score 70.70

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 1090
variable_sites: 59
pi: 0.0179
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TCACAAGCGGTATTCAAGCT	GCAGCAATAACAGAAGCAGC	45.0	50.0	57.5	58.1	1058-1221 bp	1.0	83.5
atpI-atpH_p2	GCTGCGGCTTATATAGGTGA	GCAGCAATAACAGAAGCAGC	50.0	50.0	57.3	58.1	1020-1183 bp	1.0	81.4
atpI-atpH_p3	ACAAGCGGTATTCAAGCTCT	GCAGCAATAACAGAAGCAGC	45.0	50.0	57.2	58.1	1056-1219 bp	1.0	81.1
atpI-atpH_p4	TCACAAGCGGTATTCAAGCT	AGGTCCAATAGAAGCAAGCC	45.0	50.0	57.5	57.6	1092-1255 bp	1.0	80.7
atpI-atpH_p5	TCACAAGCGGTATTCAAGCT	GTCCAATAGAAGCAAGCCCT	45.0	50.0	57.5	57.6	1090-1253 bp	1.0	80.7