Hotspot

petN-trnC-GCA

LSC · 32642-33713 · 1072 bp

Back to Glycine

Pi 0.0248

MarkerSeek score 62.40

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1072
variable_sites: 81
pi: 0.0248
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	TTAAAGCAGCCCAAGCAAGA	TTTCCCCAGTTCAAATCCGG	45.0	50.0	58.0	58.1	1135-1161 bp	1.0	86.6
petN-trnC-GCA_p2	CAGCCCAAGCAAGACTTACT	TTTCCCCAGTTCAAATCCGG	50.0	50.0	57.8	58.1	1129-1155 bp	1.0	85.2
petN-trnC-GCA_p3	TTAAAGCAGCCCAAGCAAGA	GGGGGACTGCAAATCCTTTT	45.0	50.0	58.0	58.4	1153-1179 bp	1.0	84.3
petN-trnC-GCA_p4	TTAAAGCAGCCCAAGCAAGA	TATCTATTTGGCGGCATGGC	45.0	50.0	58.0	58.5	1186-1212 bp	1.0	83.2
petN-trnC-GCA_p5	CAGCCCAAGCAAGACTTACT	GGGGGACTGCAAATCCTTTT	50.0	50.0	57.8	58.4	1147-1173 bp	1.0	82.9