Hotspot

trnY-GUA-trnD-GUC

LSC · 30830-31284 · 455 bp

Back to Glycine

Pi 0.0264

MarkerSeek score 64.70

Primer available yes

Label trnY-GUA-trnD-GUC

Feature Metadata

label_name: trnY-GUA-trnD-GUC
feature_type: igs
region: LSC
length_bp: 455
variable_sites: 30
pi: 0.0264
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnD-GUC_p1	TGGCAATATGTCTACGCTGG	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.8	58.5	514-527 bp	1.0	82.6
trnY-GUA-trnD-GUC_p2	GGCAATATGTCTACGCTGGT	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.8	58.5	513-526 bp	1.0	82.6
trnY-GUA-trnD-GUC_p3	GGGGACGGACTGTAAATTCG	CGGTGCTCTGACCAATTGAA	55.0	50.0	58.4	58.5	535-548 bp	1.0	81.3
trnY-GUA-trnD-GUC_p4	GGGACGGACTGTAAATTCGT	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.6	58.5	534-547 bp	1.0	81.2
trnY-GUA-trnD-GUC_p5	CGGACTGTAAATTCGTTGGC	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.5	58.5	530-543 bp	1.0	80.1