Hotspot

trnT-GGU-trnE-UUC

LSC · 29770-30606 · 837 bp

Back to Glycine

Pi 0.0264
MarkerSeek score 73.80
Primer available yes
Label trnT-GGU-trnE-UUC

Feature Metadata

label_name
trnT-GGU-trnE-UUC
feature_type
igs
region
LSC
length_bp
837
variable_sites
64
pi
0.0264
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnE-UUC_p1 GAACCGATGACTTACGCCTT GCTGCCTCCTTGAAAGAGAG 50.0 55.0 58.0 58.3 861-954 bp 1.0 87.4
trnT-GGU-trnE-UUC_p2 GAACCGATGACTTACGCCTT TGTCCTGAACCACTAGACGA 50.0 50.0 58.0 57.7 840-933 bp 1.0 87.4
trnT-GGU-trnE-UUC_p3 CCATGGCGTTACTCTACCAC GCTGCCTCCTTGAAAGAGAG 55.0 55.0 58.1 58.3 840-933 bp 1.0 87.1
trnT-GGU-trnE-UUC_p4 CCATGGCGTTACTCTACCAC TGTCCTGAACCACTAGACGA 55.0 50.0 58.1 57.7 819-912 bp 1.0 87.1
trnT-GGU-trnE-UUC_p5 TGGCGTTACTCTACCACTGA GCTGCCTCCTTGAAAGAGAG 50.0 55.0 58.1 58.3 837-930 bp 1.0 86.9