Hotspot

ndhF

SSC · 102384-104603 · 2220 bp

Back to Glyceria

Pi 0.0144

MarkerSeek score 52.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 127
pi: 0.0144
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AACACCCATCAATGCATGGT	TAAGCAAGAGCCGCATCTTT	45.0	45.0	58.1	57.9	2761-2824 bp	0.433333	60.0
ndhF_p2	ACCCATCAATGCATGGTGTT	TAAGCAAGAGCCGCATCTTT	45.0	45.0	58.1	57.9	2758-2821 bp	0.433333	60.0
ndhF_p3	AATCGCGGAAAACCGAAGTA	TAAGCAAGAGCCGCATCTTT	45.0	45.0	57.9	57.9	2655-2718 bp	0.433333	60.0
ndhF_p4	ATCGCGGAAAACCGAAGTAA	TAAGCAAGAGCCGCATCTTT	45.0	45.0	57.9	57.9	2654-2717 bp	0.433333	60.0
ndhF_p5	ACCCATCAATGCATGGTGTT	CCTATAAGCAAGAGCCGCAT	45.0	50.0	58.1	57.8	2762-2825 bp	0.433333	59.4