Hotspot

rpl32-trnL

SSC · 105618-106259 · 642 bp

Back to Glyceria

Pi 0.0291

MarkerSeek score 73.70

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 642
variable_sites: 74
pi: 0.0291
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	GGATAAGACCCCGTACCGTA	TTCGAACCGAGATGCTTGAG	55.0	50.0	58.0	58.0	1141-1271 bp	0.966667	89.8
rpl32-trnL_p2	GGATAAGACCCCGTACCGTA	GGATTCGAACCGAGATGCTT	55.0	50.0	58.0	58.1	1144-1274 bp	0.966667	89.4
rpl32-trnL_p3	GGATAAGACCCCGTACCGTA	GATCCGCACAAAAGAGTTGC	55.0	50.0	58.0	58.0	1693-1823 bp	0.966667	88.0
rpl32-trnL_p4	AAAGGGTTTTTCTGCGCAAC	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.1	58.0	699-869 bp	0.7	77.8
rpl32-trnL_p5	CAAAGGGTTTTTCTGCGCAA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.1	58.0	700-870 bp	0.7	77.7