Hotspot

ndhF-rpl32

SSC · 104604-105425 · 822 bp

Back to Glyceria

Pi 0.0268

MarkerSeek score 74.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 822
variable_sites: 97
pi: 0.0268
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACACATTCCCACAAGTTCCC	GTTGCGCAGAAAAACCCTTT	50.0	45.0	58.0	58.1	1423-1614 bp	1.0	90.0
ndhF-rpl32_p2	ACACATTCCCACAAGTTCCC	TTGCGCAGAAAAACCCTTTG	50.0	45.0	58.0	58.1	1422-1613 bp	1.0	90.0
ndhF-rpl32_p3	CACATTCCCACAAGTTCCCA	GTTGCGCAGAAAAACCCTTT	50.0	45.0	58.0	58.1	1422-1613 bp	1.0	90.0
ndhF-rpl32_p4	TACGTGAAGAGGGAATTGCG	GTTGCGCAGAAAAACCCTTT	50.0	45.0	58.0	58.1	1696-1887 bp	1.0	89.5
ndhF-rpl32_p5	TACGTGAAGAGGGAATTGCG	TTGCGCAGAAAAACCCTTTG	50.0	45.0	58.0	58.1	1695-1886 bp	1.0	89.5