Hotspot

rbcL-psaI

LSC · 56607-58039 · 1433 bp

Back to Glyceria

Pi 0.015

MarkerSeek score 78.00

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1433
variable_sites: 102
pi: 0.015
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	TCGAGTTCGAGCCAGTAGAT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1407-1697 bp	1.0	88.7
rbcL-psaI_p2	TCGAGTTCGAGCCAGTAGAT	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	1413-1703 bp	1.0	88.1
rbcL-psaI_p3	AATGGAGTCCTGAACTTGCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	1458-1748 bp	1.0	87.2
rbcL-psaI_p4	CGCAGCTTGTGAAGTATGGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.3	58.0	1439-1729 bp	1.0	86.7
rbcL-psaI_p5	AATGGAGTCCTGAACTTGCC	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.8	57.9	1464-1754 bp	1.0	86.6