Hotspot

ndhC-trnV

LSC · 50592-51451 · 860 bp

Back to Glyceria

Pi 0.0184

MarkerSeek score 75.90

Primer available yes

Label ndhC-trnV

Feature Metadata

label_name: ndhC-trnV
feature_type: igs
region: LSC
length_bp: 860
variable_sites: 63
pi: 0.0184
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV_p1	CTAACCGGGGCTAAAAGTCC	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.0	57.9	882-1020 bp	0.933333	85.2
ndhC-trnV_p2	CTAACCGGGGCTAAAAGTCC	CGGTTCGAATCCGTATAGCC	55.0	55.0	58.0	58.3	883-1021 bp	0.933333	83.4
ndhC-trnV_p3	TAACCGGGGCTAAAAGTCCT	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.3	57.9	881-1019 bp	0.933333	82.8
ndhC-trnV_p4	CACTAACCGGGGCTAAAAGT	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.5	57.9	884-1022 bp	0.933333	81.6
ndhC-trnV_p5	TAACCGGGGCTAAAAGTCCT	CGGTTCGAATCCGTATAGCC	50.0	55.0	58.3	58.3	882-1020 bp	0.933333	80.9