Hotspot

trnF-ndhJ

LSC · 48433-48920 · 488 bp

Back to Glyceria

Pi 0.0189

MarkerSeek score 66.80

Primer available yes

Label trnF-ndhJ

Feature Metadata

label_name: trnF-ndhJ
feature_type: igs
region: LSC
length_bp: 488
variable_sites: 36
pi: 0.0189
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CCTAATGCCCGAAAGTTGGA	50.0	50.0	58.1	57.9	606-644 bp	1.0	86.2
trnF-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TCCTAATGCCCGAAAGTTGG	50.0	50.0	58.1	57.9	607-645 bp	1.0	86.1
trnF-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	587-625 bp	1.0	85.7
trnF-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	TCCACGCCTTAAACGTATCC	50.0	50.0	58.1	57.7	624-662 bp	1.0	84.7
trnF-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	CCACGCCTTAAACGTATCCT	50.0	50.0	58.1	57.7	623-661 bp	1.0	84.6