Hotspot

trnC-rpoB

LSC · 19124-20237 · 1114 bp

Back to Glyceria

Pi 0.0119

MarkerSeek score 66.00

Primer available yes

Label trnC-rpoB

Feature Metadata

label_name: trnC-rpoB
feature_type: igs
region: LSC
length_bp: 1114
variable_sites: 68
pi: 0.0119
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-rpoB_p1	TTATTGATAAGGCGGCACCC	CCGGGTATTGTGGACATTCC	50.0	55.0	58.0	58.3	1230-1314 bp	1.0	82.7
trnC-rpoB_p2	TTATTGATAAGGCGGCACCC	CGGGTATTGTGGACATTCCC	50.0	55.0	58.0	58.3	1229-1313 bp	1.0	82.7
trnC-rpoB_p3	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	50.0	55.0	58.1	58.3	1193-1277 bp	1.0	82.5
trnC-rpoB_p4	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	1192-1276 bp	1.0	82.5
trnC-rpoB_p5	TTATTGATAAGGCGGCACCC	AAGTTCTTCTGCCAAGCCTT	50.0	45.0	58.0	57.6	1294-1378 bp	1.0	82.1