Hotspot

trnG-trnfM

LSC · 12397-12861 · 465 bp

Back to Glyceria

Pi 0.0358

MarkerSeek score 70.50

Primer available yes

Label trnG-trnfM

Feature Metadata

label_name: trnG-trnfM
feature_type: igs
region: LSC
length_bp: 465
variable_sites: 54
pi: 0.0358
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-trnfM_p1	AGGAGAAGATACGGGTTCGA	CGAGGCTCATAACCTTGAGG	50.0	55.0	57.6	57.8	539-768 bp	1.0	84.1
trnG-trnfM_p2	AGGAGAAGATACGGGTTCGA	TTCCACCTTTTTCGTCCACC	50.0	50.0	57.6	58.3	602-832 bp	1.0	83.8
trnG-trnfM_p3	AGGAGAAGATACGGGTTCGA	CACCTTTTTCGTCCACCAGT	50.0	50.0	57.6	58.3	599-829 bp	1.0	83.8
trnG-trnfM_p4	AGGAGAAGATACGGGTTCGA	CAGTTTGGTAGCTCACGAGG	50.0	55.0	57.6	58.3	554-783 bp	1.0	83.5
trnG-trnfM_p5	AGGAGAAGATACGGGTTCGA	TTTTTCGTCCACCAGTTCCC	50.0	50.0	57.6	58.3	595-825 bp	0.9	79.0