Hotspot

trnS-psbD

LSC · 7886-8842 · 957 bp

Back to Glyceria

Pi 0.0169

MarkerSeek score 68.70

Primer available yes

Label trnS-psbD

Feature Metadata

label_name: trnS-psbD
feature_type: igs
region: LSC
length_bp: 957
variable_sites: 66
pi: 0.0169
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-psbD_p1	ATTAGCAATCCGCCGCTTTA	GACAAAAACGAAACGGTCCC	45.0	50.0	58.0	57.9	1042-1115 bp	1.0	87.6
trnS-psbD_p2	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.0	58.2	1041-1114 bp	1.0	86.6
trnS-psbD_p3	ATTAGCAATCCGCCGCTTTA	CCGACAAAAACGAAACGGTC	45.0	50.0	58.0	58.3	1044-1117 bp	1.0	86.4
trnS-psbD_p4	ATTAGCAATCCGCCGCTTTA	CGACAAAAACGAAACGGTCC	45.0	50.0	58.0	58.3	1043-1116 bp	1.0	86.4
trnS-psbD_p5	GAGCGGAAAGAGAGGGATTC	GACAAAAACGAAACGGTCCC	55.0	50.0	57.8	57.9	1093-1166 bp	1.0	85.9