Hotspot

trnK-rps16

LSC · 3948-4493 · 546 bp

Back to Glyceria

Pi 0.0282

MarkerSeek score 68.40

Primer available yes

Label trnK-rps16

Feature Metadata

label_name: trnK-rps16
feature_type: igs
region: LSC
length_bp: 546
variable_sites: 58
pi: 0.0282
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-rps16_p1	TGAGGATCAGTCGTGGTCTT	TCGAGCCGTATGAGGAGAAA	50.0	50.0	58.1	58.2	940-990 bp	1.0	86.9
trnK-rps16_p2	TGAGGATCAGTCGTGGTCTT	TTTCTCGAGCCGTATGAGGA	50.0	50.0	58.1	58.2	944-994 bp	1.0	86.9
trnK-rps16_p3	ACGTCTAATTAGAGCGGGGA	TCGAGCCGTATGAGGAGAAA	50.0	50.0	57.9	58.2	1127-1176 bp	1.0	86.7
trnK-rps16_p4	ACGTCTAATTAGAGCGGGGA	TTTCTCGAGCCGTATGAGGA	50.0	50.0	57.9	58.2	1131-1180 bp	1.0	86.7
trnK-rps16_p5	ACGTCTAATTAGAGCGGGGA	CCTTTGAAGTCTGTCAGGCA	50.0	50.0	57.9	57.7	1259-1308 bp	1.0	85.9