Hotspot

ndhD

SSC · 118134-119654 · 1521 bp

Back to Glochidion

Pi 0.00142

MarkerSeek score 54.40

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1521
variable_sites: 4
pi: 0.00142
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TGAGTGTAGGTCGGTTTTGA	CAAAAACCCGTGCTCGAAAA	45.0	45.0	56.4	57.8	1645 bp	1.0	66.0
ndhD_p2	TGAGTGTAGGTCGGTTTTGA	AAAACCCGTGCTCGAAAAGA	45.0	45.0	56.4	58.3	1643 bp	1.0	65.0
ndhD_p3	TGAGTGTAGGTCGGTTTTGA	AAACCCGTGCTCGAAAAGAA	45.0	45.0	56.4	58.3	1642 bp	1.0	65.0
ndhD_p4	TGAGTGTAGGTCGGTTTTGA	AACCCGTGCTCGAAAAGAAA	45.0	45.0	56.4	58.3	1641 bp	1.0	65.0
ndhD_p5	TGAGTGTAGGTCGGTTTTGA	ACCCGTGCTCGAAAAGAAAA	45.0	45.0	56.4	58.3	1640 bp	1.0	65.0