Hotspot

cemA

LSC · 61787-62476 · 690 bp

Back to Glochidion

Pi 0.00314

MarkerSeek score 54.90

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 690
variable_sites: 4
pi: 0.00314
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	TTCATAGCTTCCAGGCCTTG	ACAAACATTCTAATTGGGTCGG	50.0	40.9	57.6	57.0	823-825 bp	1.0	52.6
cemA_p2	TTCATAGCTTCCAGGCCTTG	CAAACATTCTAATTGGGTCGGT	50.0	40.9	57.6	57.0	822-824 bp	1.0	52.6
cemA_p3	GTTCATAGCTTCCAGGCCTT	ACAAACATTCTAATTGGGTCGG	50.0	40.9	57.6	57.0	824-826 bp	1.0	52.6
cemA_p4	GTTCATAGCTTCCAGGCCTT	CAAACATTCTAATTGGGTCGGT	50.0	40.9	57.6	57.0	823-825 bp	1.0	52.6
cemA_p5	TCATAGCTTCCAGGCCTTGT	CAAACATTCTAATTGGGTCGGT	50.0	40.9	58.7	57.0	821-823 bp	1.0	50.3