Hotspot

ndhB-trnL-CAA

IRa · 146424-146990 · 567 bp

Back to Glochidion

Pi 0.000882

MarkerSeek score 40.30

Primer available yes

Label ndhB-trnL-CAA

Feature Metadata

label_name: ndhB-trnL-CAA
feature_type: igs
region: IRa
length_bp: 567
variable_sites: 1
pi: 0.000882
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-trnL-CAA_p1	CAGGAATCTCAATGAACCCGA	GTCTCGCGTGTCTACCATTT	47.6	50.0	57.7	58.0	652 bp	1.0	69.9
ndhB-trnL-CAA_p2	CAGGAATCTCAATGAACCCGA	CTCGCGTGTCTACCATTTCA	47.6	50.0	57.7	58.0	650 bp	1.0	69.9
ndhB-trnL-CAA_p3	CAGGAATCTCAATGAACCCGA	TACCATTTCACCACCAAGGC	47.6	50.0	57.7	58.1	640 bp	1.0	69.3
ndhB-trnL-CAA_p4	ACCAGGAATCTCAATGAACCC	GTCTCGCGTGTCTACCATTT	47.6	50.0	57.6	58.0	654 bp	1.0	69.1
ndhB-trnL-CAA_p5	ACCAGGAATCTCAATGAACCC	CTCGCGTGTCTACCATTTCA	47.6	50.0	57.6	58.0	652 bp	1.0	69.1