Hotspot

rpl32-trnL-UAG

SSC · 115701-116745 · 1045 bp

Back to Glochidion

Pi 0.00144

MarkerSeek score 60.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1045
variable_sites: 3
pi: 0.00144
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGGGTAGCGTTGAAAGCTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.0	58.0	1152-1160 bp	1.0	80.1
rpl32-trnL-UAG_p2	TTGGGTAGCGTTGAAAGCTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.0	58.0	1153-1161 bp	1.0	80.1
rpl32-trnL-UAG_p3	TGGGTAGCGTTGAAAGCTTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.0	58.0	1153-1161 bp	1.0	80.1
rpl32-trnL-UAG_p4	TTGGGTAGCGTTGAAAGCTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.0	58.0	1154-1162 bp	1.0	80.1
rpl32-trnL-UAG_p5	TGGGTAGCGTTGAAAGCTTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.0	57.9	1162-1170 bp	1.0	79.2