Hotspot

petA-psbJ

LSC · 63653-64563 · 911 bp

Back to Glochidion

Pi 0.0115

MarkerSeek score 57.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 911
variable_sites: 21
pi: 0.0115
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CATTACGTGTCCAAGGCCTT	ACTGGTATTCCTGTGATCGG	50.0	50.0	58.2	56.7	1102-1112 bp	1.0	69.9
petA-psbJ_p2	CATTACGTGTCCAAGGCCTT	CTGGTATTCCTGTGATCGGT	50.0	50.0	58.2	56.7	1101-1111 bp	1.0	69.9
petA-psbJ_p3	GACCCATTACGTGTCCAAGG	ACTGGTATTCCTGTGATCGG	55.0	50.0	58.3	56.7	1106-1116 bp	1.0	69.2
petA-psbJ_p4	GACCCATTACGTGTCCAAGG	CTGGTATTCCTGTGATCGGT	55.0	50.0	58.3	56.7	1105-1115 bp	1.0	69.2
petA-psbJ_p5	TTACGTGTCCAAGGCCTTTT	CTGGTATTCCTGTGATCGGT	45.0	50.0	57.6	56.7	1099-1109 bp	1.0	68.5