Hotspot

trnM-CAU-atpE

LSC · 52696-52942 · 247 bp

Back to Glochidion

Pi 0.0081

MarkerSeek score 37.20

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 247
variable_sites: 4
pi: 0.0081
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	GGCAGGAGTCATTGGTTCAA	ACTTGAGGAAAGCAGAAGGC	50.0	50.0	58.1	58.1	380 bp	1.0	79.3
trnM-CAU-atpE_p2	TTGCTTTCATACGGCAGGAG	ACTTGAGGAAAGCAGAAGGC	50.0	50.0	58.3	58.1	392 bp	1.0	77.9
trnM-CAU-atpE_p3	GGCAGGAGTCATTGGTTCAA	CTCTCAGACGAGCTAGAGCA	50.0	55.0	58.1	58.3	332 bp	1.0	77.5
trnM-CAU-atpE_p4	CATACGGCAGGAGTCATTGG	ACTTGAGGAAAGCAGAAGGC	55.0	50.0	58.4	58.1	385 bp	1.0	76.7
trnM-CAU-atpE_p5	ATTGCTTTCATACGGCAGGA	ACTTGAGGAAAGCAGAAGGC	45.0	50.0	57.6	58.1	393 bp	1.0	76.7