Hotspot

psbM-trnD-GUC

LSC · 29392-30536 · 1145 bp

Back to Glochidion

Pi 0.00175
MarkerSeek score 60.90
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1145
variable_sites
4
pi
0.00175
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 TTGACTCACGGTTTTTACGT TCCCGGGGATTGTAGTTCAA 40.0 50.0 55.6 58.3 1311-1320 bp 1.0 58.6
psbM-trnD-GUC_p2 TTGACTCACGGTTTTTACGT TTCCCGGGGATTGTAGTTCA 40.0 50.0 55.6 58.3 1312-1321 bp 1.0 58.6
psbM-trnD-GUC_p3 ACTTTGACTCACGGTTTTTACG TCCCGGGGATTGTAGTTCAA 40.9 50.0 57.5 58.3 1314-1323 bp 1.0 57.9
psbM-trnD-GUC_p4 CTTTGACTCACGGTTTTTACGT TCCCGGGGATTGTAGTTCAA 40.9 50.0 57.5 58.3 1313-1322 bp 1.0 57.9
psbM-trnD-GUC_p5 CTTTGACTCACGGTTTTTACGT TTCCCGGGGATTGTAGTTCA 40.9 50.0 57.5 58.3 1314-1323 bp 1.0 57.9