Hotspot

rps16-trnQ-UUG

LSC · 6628-7563 · 936 bp

Back to Glochidion

Pi 0.00231
MarkerSeek score 65.50
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
936
variable_sites
4
pi
0.00231
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CCACAACCGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1030-1289 bp 1.0 82.8
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1009-1268 bp 1.0 82.6
rps16-trnQ-UUG_p3 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1020-1279 bp 1.0 82.0
rps16-trnQ-UUG_p4 ATCCACAACCGATCATGTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.7 58.1 1032-1291 bp 1.0 81.0
rps16-trnQ-UUG_p5 CCACAACCGATCATGTCCTT TTCGGAGGTTCGAATCCTTC 50.0 50.0 57.9 57.3 1034-1293 bp 1.0 78.0