Hotspot

ycf1

SSC · 122665-128028 · 5364 bp

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Pi 0.00922

MarkerSeek score 45.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5364
variable_sites: 73
pi: 0.00922
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTCCTTAAAAACGGGTTTAAAA	TCTGTATGAAACCTTGGCACA	27.3	42.9	52.7	57.5	173 bp	1.0	43.2
ycf1_p2	TTTCCTTAAAAACGGGTTTAAA	TCTGTATGAAACCTTGGCACA	27.3	42.9	52.7	57.5	174 bp	1.0	43.2
ycf1_p3	TTTCCTTAAAAACGGGTTTAAAA	TCTGTATGAAACCTTGGCACA	26.1	42.9	53.5	57.5	174 bp	1.0	43.2
ycf1_p4	ATTTCCTTAAAAACGGGTTTAA	TCTGTATGAAACCTTGGCACA	27.3	42.9	52.5	57.5	175 bp	1.0	43.2
ycf1_p5	ATTTCCTTAAAAACGGGTTTAAA	TCTGTATGAAACCTTGGCACA	26.1	42.9	53.3	57.5	175 bp	1.0	43.2