Hotspot

ndhA

SSC · 118527-120684 · 2158 bp

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Pi 0.012

MarkerSeek score 54.80

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2158
variable_sites: 39
pi: 0.012
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACTCGTTTCTTTCTCTTGTTTGAG	TGGGAGAAGTCGATCGTTGA	37.5	50.0	57.6	58.5	2230-2241 bp	1.0	43.3
ndhA_p2	ACTCGTTTCTTTCTCTTGTTTGA	TGGGAGAAGTCGATCGTTGA	34.8	50.0	56.6	58.5	2230-2241 bp	1.0	43.1
ndhA_p3	ACTCGTTTCTTTCTCTTGTTTGAG	ATGGGAGAAGTCGATCGTTG	37.5	50.0	57.6	57.4	2231-2242 bp	1.0	42.3
ndhA_p4	ACTCGTTTCTTTCTCTTGTTTGA	ATGGGAGAAGTCGATCGTTG	34.8	50.0	56.6	57.4	2231-2242 bp	1.0	42.1
ndhA_p5	ACTCGTTTCTTTCTCTTGTTTGAG	GGGAGAAGTCGATCGTTGAA	37.5	50.0	57.6	57.4	2229-2240 bp	1.0	41.8