Hotspot

rpl20-rps12

LSC · 69229-70001 · 773 bp

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Pi 0.0173

MarkerSeek score 65.00

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 773
variable_sites: 20
pi: 0.0173
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCGAGCTATATATCCGCGT	AAATGTCACGAAATCCCCCG	50.0	50.0	58.0	58.5	874-881 bp	1.0	78.0
rpl20-rps12_p2	CTTGAATGAGCCCCTCGAAA	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	929-936 bp	1.0	77.2
rpl20-rps12_p3	TTGAATGAGCCCCTCGAAAG	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	928-935 bp	1.0	77.2
rpl20-rps12_p4	TCCGAGCTATATATCCGCGT	CAGCGTCGAGGAACATGTAC	50.0	55.0	58.0	58.7	837-844 bp	1.0	76.5
rpl20-rps12_p5	TTGAATGAGCCCCTCGAAAG	CAGCGTCGAGGAACATGTAC	50.0	55.0	57.9	58.7	891-898 bp	1.0	75.7