Hotspot

accD-psaI

LSC · 59445-60217 · 773 bp

Back to Globularia

Pi 0.0403

MarkerSeek score 73.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 773
variable_sites: 45
pi: 0.0403
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CCAGAAGGGCTTATTCGACC	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	935-955 bp	1.0	85.8
accD-psaI_p2	CGGCTAGTTTTGGCATGTTG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1071-1091 bp	1.0	85.2
accD-psaI_p3	CCAGAAGGGCTTATTCGACC	TTTGTTTTCGAGACCCCGTT	55.0	45.0	58.0	58.0	1453-1472 bp	1.0	84.0
accD-psaI_p4	CGGCTAGTTTTGGCATGTTG	TTTGTTTTCGAGACCCCGTT	50.0	45.0	58.0	58.0	1589-1608 bp	1.0	83.8
accD-psaI_p5	CTTTCGATCGATCCCGGTAC	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	1510-1530 bp	1.0	83.5