Hotspot

rpoB-trnC-GCA

LSC · 26888-28128 · 1241 bp

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Pi 0.00694

MarkerSeek score 64.10

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1241
variable_sites: 12
pi: 0.00694
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCATTTGCATTCCCCAGCAT	CCGGATTTGAACTGGGGAAA	45.0	50.0	58.1	58.1	1274-1323 bp	1.0	82.1
rpoB-trnC-GCA_p2	TTCCCTCATTTGCATTCCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.1	58.1	1279-1328 bp	1.0	82.0
rpoB-trnC-GCA_p3	TCATTTGCATTCCCCAGCAT	AAAAGGATTTGCAGTCCCCC	45.0	50.0	58.1	58.4	1256-1305 bp	1.0	79.9
rpoB-trnC-GCA_p4	TTCCCTCATTTGCATTCCCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.1	58.4	1261-1310 bp	1.0	79.9
rpoB-trnC-GCA_p5	CTCATTTGCATTCCCCAGCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.8	58.1	1275-1324 bp	1.0	76.6