Hotspot

ndhG-ndhI

SSC · 125517-125828 · 312 bp

Back to Globba

Pi 0.036
MarkerSeek score 62.50
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
312
variable_sites
22
pi
0.036
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 GTGTTGGTCCTGGTAAATCCA CCCTTGATTCAAGAACAATTACCA 47.6 37.5 57.9 57.4 311-375 bp 1.0 48.9
ndhG-ndhI_p2 TGTGTTGGTCCTGGTAAATCC CCCTTGATTCAAGAACAATTACCA 47.6 37.5 57.9 57.4 312-376 bp 1.0 48.9
ndhG-ndhI_p3 GTGTTGGTCCTGGTAAATCCA TCCCTTGATTCAAGAACAATTACC 47.6 37.5 57.9 57.2 312-376 bp 1.0 48.9
ndhG-ndhI_p4 TGTGTTGGTCCTGGTAAATCC TCCCTTGATTCAAGAACAATTACC 47.6 37.5 57.9 57.2 313-377 bp 1.0 48.9
ndhG-ndhI_p5 GTGTTGGTCCTGGTAAATCCA CCCTTGATTCAAGAACAATTACCAA 47.6 36.0 57.9 58.0 311-375 bp 1.0 48.9