Hotspot

psaC-ndhE

SSC · 124026-124466 · 441 bp

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Pi 0.0485
MarkerSeek score 81.70
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
441
variable_sites
46
pi
0.0485
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 CGTCCCACGGTATCATTTCC CGGAAGCTGCTATTGGACTT 55.0 50.0 58.4 58.0 590-930 bp 1.0 85.6
psaC-ndhE_p2 GGCAAGCTCGTACACATTGA CGGAAGCTGCTATTGGACTT 50.0 50.0 58.6 58.0 557-897 bp 1.0 84.8
psaC-ndhE_p3 GTCCCACGGTATCATTTCCA CGGAAGCTGCTATTGGACTT 50.0 50.0 57.3 58.0 589-929 bp 1.0 83.4
psaC-ndhE_p4 GCAAGCTCGTACACATTGAG CGGAAGCTGCTATTGGACTT 50.0 50.0 57.2 58.0 556-896 bp 1.0 82.9
psaC-ndhE_p5 TAGGGCAAGCTCGTACACAT CGGAAGCTGCTATTGGACTT 50.0 50.0 58.8 58.0 560-900 bp 1.0 82.8