Hotspot

ccsA-ndhD

SSC · 121698-122149 · 452 bp

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Pi 0.0405
MarkerSeek score 52.30
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
452
variable_sites
28
pi
0.0405
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.69
markerseek_score
52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 ACTGAAATGCACCAATCCGT GGATTCTGGACCACGAGAAC 45.0 55.0 58.1 58.0 1067-1233 bp 1.0 86.6
ccsA-ndhD_p2 CCACGTCGGGTCTTTTAACT GGATTCTGGACCACGAGAAC 50.0 55.0 57.8 58.0 1084-1250 bp 1.0 86.0
ccsA-ndhD_p3 ATATTGGAGTTGGGACCCCA GGATTCTGGACCACGAGAAC 50.0 55.0 58.0 58.0 666-832 bp 0.857143 81.6
ccsA-ndhD_p4 ATATTGGAGTTGGGACCCCA CCTCTTTCGGTCAACGGAAT 50.0 50.0 58.0 57.9 1150-1316 bp 0.857143 79.8
ccsA-ndhD_p5 ATATTGGAGTTGGGACCCCA CCATAACTGGAATCGGGCTC 50.0 55.0 58.0 58.1 1066-1232 bp 0.857143 79.8