Hotspot

ndhF-rpl32

SSC · 118764-119432 · 669 bp

Back to Globba

Pi 0.0202
MarkerSeek score 63.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
669
variable_sites
30
pi
0.0202
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGAAGAAAAGTT AGAAAAAGCCTTTACTGCAGC 40.0 42.9 53.3 57.3 801-838 bp 1.0 45.6
ndhF-rpl32_p2 CTGGAAGTGGAAGAAAAGTT GAAAAAGCCTTTACTGCAGCT 40.0 42.9 53.3 57.3 800-837 bp 1.0 45.6
ndhF-rpl32_p3 CTGGAAGTGGAAGAAAAGTT AAAAAGCCTTTACTGCAGCT 40.0 40.0 53.3 56.1 799-836 bp 1.0 45.6
ndhF-rpl32_p4 CTGGAAGTGGAAGAAAAGTT AAGAAAAAGCCTTTACTGCAGC 40.0 40.9 53.3 58.0 802-839 bp 1.0 45.6
ndhF-rpl32_p5 CTGGAAGTGGAAGAAAAGTT GAAAAAGCCTTTACTGCAGC 40.0 45.0 53.3 55.8 800-837 bp 1.0 45.6