Hotspot

ycf1-ndhF

SSC · 116502-116543 · 42 bp

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Pi 0.256
MarkerSeek score 61.60
Primer available yes
Label ycf1-ndhF

Feature Metadata

label_name
ycf1-ndhF
feature_type
igs
region
SSC
length_bp
42
variable_sites
16
pi
0.256
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.55
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-ndhF_p1 CGCGGTACCCATCCTTTTAT CAATGGGGTTGTTGTTGCAA 50.0 45.0 57.8 57.7 529-586 bp 1.0 85.7
ycf1-ndhF_p2 CAATATCGCGGTACCCATCC CAATGGGGTTGTTGTTGCAA 55.0 45.0 58.3 57.7 535-592 bp 1.0 85.4
ycf1-ndhF_p3 CGCGGTACCCATCCTTTTAT GGGGTTGTTGTTGCAAGTTT 50.0 45.0 57.8 57.6 525-582 bp 1.0 85.2
ycf1-ndhF_p4 CGCGGTACCCATCCTTTTAT ATGGGAGGTGGACGAATTTC 50.0 50.0 57.8 57.6 480-537 bp 1.0 84.9
ycf1-ndhF_p5 CAATATCGCGGTACCCATCC GGGGTTGTTGTTGCAAGTTT 55.0 45.0 58.3 57.6 531-588 bp 1.0 84.9