Hotspot

psbT-psbN

LSC · 76766-76824 · 59 bp

Back to Globba

Pi 0.042
MarkerSeek score 67.30
Primer available yes
Label psbT-psbN

Feature Metadata

label_name
psbT-psbN
feature_type
igs
region
LSC
length_bp
59
variable_sites
6
pi
0.042
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbT-psbN_p1 TTTCGAGAACCACCTAAGGT ATCCATTCGAAGAACACGGG 45.0 50.0 56.1 58.0 127-132 bp 1.0 68.9
psbT-psbN_p2 TTTTCGAGAACCACCTAAGGT ATCCATTCGAAGAACACGGG 42.9 50.0 56.9 58.0 128-133 bp 1.0 67.1
psbT-psbN_p3 ACGATCGAATCTATGGAAGCA ATCCATTCGAAGAACACGGG 42.9 50.0 57.0 58.0 205-210 bp 1.0 66.4
psbT-psbN_p4 TTTCGAGAACCACCTAAGGT TTCGAAGAACACGGGGACTA 45.0 50.0 56.1 58.4 122-127 bp 1.0 66.3
psbT-psbN_p5 TTTCGAGAACCACCTAAGGT GGGTACGCCTTATATACCGC 45.0 55.0 56.1 57.7 176-181 bp 1.0 65.7