Hotspot

psbE-petL

LSC · 66868-67903 · 1036 bp

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Pi 0.0141
MarkerSeek score 61.10
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1036
variable_sites
35
pi
0.0141
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA AAGCAGCCAGTAGAAAACCG 50.0 50.0 57.9 58.5 1183-1429 bp 1.0 78.7
psbE-petL_p2 CAACCCGCAATGAATAGGGA GCAGCCAGTAGAAAACCGAA 50.0 50.0 57.9 58.5 1181-1427 bp 1.0 78.6
psbE-petL_p3 ACTGACGAATAACCAACCCG AAGCAGCCAGTAGAAAACCG 50.0 50.0 57.9 58.5 1196-1442 bp 1.0 78.3
psbE-petL_p4 ACTGACGAATAACCAACCCG GCAGCCAGTAGAAAACCGAA 50.0 50.0 57.9 58.5 1194-1440 bp 1.0 78.3
psbE-petL_p5 ACGAATAACCAACCCGCAAT AAGCAGCCAGTAGAAAACCG 45.0 50.0 58.2 58.5 1192-1438 bp 1.0 77.7