Hotspot

psbC-trnS-UGA

LSC · 36846-36994 · 149 bp

Back to Globba

Pi 0.041
MarkerSeek score 65.30
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
149
variable_sites
13
pi
0.041
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 CTTTCCATGACCCCTCTTCG GGGTTCGAATCCCTCTCTCT 55.0 55.0 58.0 58.2 193-202 bp 1.0 85.7
psbC-trnS-UGA_p2 CCTGTTCTTTCCATGACCCC GGGTTCGAATCCCTCTCTCT 55.0 55.0 58.2 58.2 199-208 bp 1.0 84.5
psbC-trnS-UGA_p3 GACCGTGATTTGGAACCTGT GGGTTCGAATCCCTCTCTCT 50.0 55.0 58.1 58.2 214-223 bp 1.0 84.4
psbC-trnS-UGA_p4 TGACCGTGATTTGGAACCTG GGGTTCGAATCCCTCTCTCT 50.0 55.0 58.1 58.2 215-224 bp 1.0 84.3
psbC-trnS-UGA_p5 CATTTGTGGCATGCAGGAAG GGGTTCGAATCCCTCTCTCT 50.0 55.0 58.0 58.2 271-280 bp 1.0 83.8