Hotspot

atpH-atpI

LSC · 13943-15037 · 1095 bp

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Pi 0.0181
MarkerSeek score 65.10
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1095
variable_sites
44
pi
0.0181
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT CCGCGGCTTATATAGGTGAA 45.0 50.0 58.0 57.3 1168-1611 bp 1.0 80.4
atpH-atpI_p2 TACCTTCTACAGCTTGGCCT CCGCGGCTTATATAGGTGAA 50.0 50.0 58.0 57.3 1251-1694 bp 1.0 80.2
atpH-atpI_p3 ATAACGGAAGCGGCAGAAAT GGTGAATCCATGGAAGGTCA 45.0 50.0 58.0 57.2 1154-1597 bp 1.0 79.8
atpH-atpI_p4 TACCTTGACCAACTCCAGGT CCGCGGCTTATATAGGTGAA 50.0 50.0 57.9 57.3 1224-1667 bp 1.0 79.7
atpH-atpI_p5 TACCTTCTACAGCTTGGCCT GGTGAATCCATGGAAGGTCA 50.0 50.0 58.0 57.2 1237-1680 bp 1.0 79.6