Hotspot

rps16-trnQ-UUG

LSC · 6005-7013 · 1009 bp

Back to Globba

Pi 0.0239
MarkerSeek score 62.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1009
variable_sites
57
pi
0.0239
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CCACAACCGATCATGTCCTT CTTACAGCAGCTTGCCAAAC 50.0 50.0 57.9 57.9 1665-2071 bp 1.0 85.4
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA CTTACAGCAGCTTGCCAAAC 45.0 50.0 58.1 57.9 1644-2050 bp 1.0 85.3
rps16-trnQ-UUG_p3 CCACAACCGATCATGTCCTT CGGGCAATTTGGCAAAGAAA 50.0 45.0 57.9 58.1 1571-1977 bp 1.0 85.0
rps16-trnQ-UUG_p4 CCACAACCGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1103-1510 bp 0.857143 78.0
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1082-1489 bp 0.857143 77.9