Hotspot

rpl33-rps18

LSC · 76335-76645 · 311 bp

Back to Gleditsia

Pi 0.0611

MarkerSeek score 92.00

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 311
variable_sites: 37
pi: 0.0611
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 92.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	TCTGTCCCTGTTGTTGCAAA	TCAATTCGATCCCCCGATTG	45.0	50.0	57.9	57.7	413-647 bp	1.0	86.6
rpl33-rps18_p2	TTCTGTCCCTGTTGTTGCAA	TCAATTCGATCCCCCGATTG	45.0	50.0	57.9	57.7	414-648 bp	1.0	86.6
rpl33-rps18_p3	ATTCTGTCCCTGTTGTTGCA	TCAATTCGATCCCCCGATTG	45.0	50.0	57.6	57.7	415-649 bp	1.0	84.8
rpl33-rps18_p4	TCTGTCCCTGTTGTTGCAAA	ATTCGATCCCCCGATTGGAT	45.0	50.0	57.9	58.6	410-644 bp	1.0	83.6
rpl33-rps18_p5	TTCTGTCCCTGTTGTTGCAA	ATTCGATCCCCCGATTGGAT	45.0	50.0	57.9	58.6	411-645 bp	1.0	83.5