Hotspot

petA-psbJ

LSC · 70578-71936 · 1359 bp

Back to Gleditsia

Pi 0.0322

MarkerSeek score 73.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1359
variable_sites: 82
pi: 0.0322
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAGAAGGTTCAATTGGCTGA	ATTCCGGATTGGGTTCATCC	42.9	50.0	57.5	57.6	852-1860 bp	1.0	73.6
petA-psbJ_p2	TGAGAAGGTTCAATTGGCTG	ATTCCGGATTGGGTTCATCC	45.0	50.0	55.9	57.6	852-1860 bp	1.0	69.1
petA-psbJ_p3	TGAGAAGGTTCAATTGGCTGAA	ATTCCGGATTGGGTTCATCC	40.9	50.0	58.2	57.6	852-1860 bp	1.0	68.3
petA-psbJ_p4	TTGAGAAGGTTCAATTGGCTGA	ATTCCGGATTGGGTTCATCC	40.9	50.0	58.2	57.6	853-1861 bp	1.0	68.3
petA-psbJ_p5	TTGAGAAGGTTCAATTGGCTG	ATTCCGGATTGGGTTCATCC	42.9	50.0	56.7	57.6	853-1861 bp	1.0	67.3