Hotspot

accD-psaI

LSC · 66736-67409 · 674 bp

Back to Gleditsia

Pi 0.118

MarkerSeek score 83.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 674
variable_sites: 141
pi: 0.118
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGGGCGTTCTGAGTGAGTTA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.4	58.0	563-1734 bp	1.0	88.8
accD-psaI_p2	AGGGCGTTCTGAGTGAGTTA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.4	57.9	569-1740 bp	1.0	88.2
accD-psaI_p3	GGGCGTTCTGAGTGAGTTAT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.3	58.0	562-1733 bp	1.0	86.4
accD-psaI_p4	TAAAGGGCGTTCTGAGTGAG	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.3	58.0	566-1737 bp	1.0	85.8
accD-psaI_p5	GGGCGTTCTGAGTGAGTTAT	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.3	57.9	568-1739 bp	1.0	85.8