Hotspot

rbcL-accD

LSC · 64075-65244 · 1170 bp

Back to Gleditsia

Pi 0.049

MarkerSeek score 80.00

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 1170
variable_sites: 101
pi: 0.049
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AATGAGGGACGTGATCTTGC	GTTCCCAAGGCCGACTAAAT	50.0	50.0	58.0	57.9	1334-1850 bp	1.0	88.2
rbcL-accD_p2	AATGAGGGACGTGATCTTGC	ATGATCGTAGTCGGGATCGT	50.0	50.0	58.0	58.1	1541-2057 bp	1.0	87.9
rbcL-accD_p3	GCCGTAGCTAATCGAGTAGC	ATGATCGTAGTCGGGATCGT	55.0	50.0	58.0	58.1	1586-2102 bp	1.0	87.7
rbcL-accD_p4	CGCTGGTACCGTAGTAGGTA	ATGATCGTAGTCGGGATCGT	55.0	50.0	58.0	58.1	1854-2370 bp	1.0	87.0
rbcL-accD_p5	GAATTGGGCGTTCCTATCGT	ATGATCGTAGTCGGGATCGT	50.0	50.0	58.1	58.1	2060-2576 bp	1.0	86.7