Pi
0.0984
MarkerSeek score
84.50
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 1330-2690 bp | 1.0 | 90.9 |
| trnT-UGU-trnL-UAA_p2 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 1338-2698 bp | 1.0 | 90.4 |
| trnT-UGU-trnL-UAA_p3 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 1362-2722 bp | 1.0 | 90.0 |
| trnT-UGU-trnL-UAA_p4 | GGAATCGAACCGATGACCAT | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 57.8 | 58.1 | 1370-2730 bp | 1.0 | 89.5 |
| trnT-UGU-trnL-UAA_p5 | GCGATGCTCTAACCTCTGAG | AGTCCGTAGCGTCTACCAAT | 55.0 | 50.0 | 57.9 | 58.2 | 1344-2704 bp | 1.0 | 89.0 |