Hotspot

trnC-GCA-petN

LSC · 31579-32887 · 1309 bp

Back to Gleditsia

Pi 0.053

MarkerSeek score 95.60

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1309
variable_sites: 145
pi: 0.053
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 95.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	1242-1425 bp	1.0	90.1
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	1303-1486 bp	1.0	88.5
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	1260-1443 bp	1.0	87.9
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.4	57.6	1321-1504 bp	1.0	86.3
trnC-GCA-petN_p5	GTATAGTTTTGGCGGCATGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.2	58.2	1294-1477 bp	1.0	84.6