Hotspot

trnG-UCC-trnR-UCU

LSC · 11694-12135 · 442 bp

Back to Gleditsia

Pi 0.0557

MarkerSeek score 78.90

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 442
variable_sites: 38
pi: 0.0557
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	CTAACGATGCGGGTTCGATT	TGTCCTATCCATTAGACAATG	50.0	38.1	58.4	52.4	499-525 bp	1.0	50.0
trnG-UCC-trnR-UCU_p2	AGCCTTCCAAGCTAACGATG	TGTCCTATCCATTAGACAATG	50.0	38.1	58.0	52.4	510-536 bp	1.0	49.8
trnG-UCC-trnR-UCU_p3	CCTAGCCTTCCAAGCTAACG	TGTCCTATCCATTAGACAATG	55.0	38.1	58.1	52.4	513-539 bp	1.0	49.8
trnG-UCC-trnR-UCU_p4	CCCTAGCCTTCCAAGCTAAC	TGTCCTATCCATTAGACAATG	55.0	38.1	57.7	52.4	514-540 bp	1.0	49.8
trnG-UCC-trnR-UCU_p5	ACCAAAGTTTCACGTTCGGT	TGTCCTATCCATTAGACAATG	45.0	38.1	58.3	52.4	580-606 bp	1.0	49.2