Hotspot

matK

LSC · 2041-3573 · 1533 bp

Back to Glechoma

Pi 0.00163

MarkerSeek score 59.80

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1533
variable_sites: 5
pi: 0.00163
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	AGTGGAAATCTCATTTTGATCT	TACTTGTCCCCGAGGTATCC	31.8	55.0	53.4	57.9	1730 bp	1.0	40.2
matK_p2	AGTGGAAATCTCATTTTGATCT	TGTCCCCGAGGTATCCATTT	31.8	50.0	53.4	58.1	1726 bp	1.0	40.2
matK_p3	AGTGGAAATCTCATTTTGATCT	TTGTCCCCGAGGTATCCATT	31.8	50.0	53.4	58.1	1727 bp	1.0	40.2
matK_p4	AGTGGAAATCTCATTTTGATCTT	TACTTGTCCCCGAGGTATCC	30.4	55.0	54.2	57.9	1730 bp	1.0	40.2
matK_p5	AGTGGAAATCTCATTTTGATCT	ACCCCCGAATCTTCTACCTT	31.8	50.0	53.4	57.7	1657 bp	1.0	40.2