Hotspot

rps15-ycf1

SSC · 121103-121494 · 392 bp

Back to Glechoma

Pi 0.0119

MarkerSeek score 63.10

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 392
variable_sites: 9
pi: 0.0119
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AGCCTCCTTATCTTAGTGGTGA	ATGGCAGTCGTTTCGGTATG	45.5	50.0	58.0	58.3	555-563 bp	1.0	63.3
rps15-ycf1_p2	AGCCTCCTTATCTTAGTGGTGA	AATGGCAGTCGTTTCGGTAT	45.5	45.0	58.0	57.6	556-564 bp	1.0	62.9
rps15-ycf1_p3	AGCCTCCTTATCTTAGTGGTGA	ATAATGGCAGTCGTTTCGGT	45.5	45.0	58.0	57.6	558-566 bp	1.0	62.9
rps15-ycf1_p4	AGCCTCCTTATCTTAGTGGTGA	GGCAGTCGTTTCGGTATGTT	45.5	50.0	58.0	58.6	553-561 bp	1.0	61.6
rps15-ycf1_p5	GCCTCCTTATCTTAGTGGTGA	ATGGCAGTCGTTTCGGTATG	47.6	50.0	56.5	58.3	554-562 bp	1.0	59.6