Hotspot

ccsA-ndhD

SSC · 113293-113555 · 263 bp

Back to Glechoma

Pi 0.0342

MarkerSeek score 65.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 263
variable_sites: 18
pi: 0.0342
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CAGCAATTGTAGCCTTTATTGGA	GTTGAAAAGGTTGAAGTTATCCT	39.1	34.8	57.4	55.1	420-432 bp	1.0	43.8
ccsA-ndhD_p2	TCAGCAATTGTAGCCTTTATTGG	GTTGAAAAGGTTGAAGTTATCCT	39.1	34.8	57.4	55.1	421-433 bp	1.0	43.8
ccsA-ndhD_p3	ACAAATTCAGCAATTGTAGCCT	GTTGAAAAGGTTGAAGTTATCCT	36.4	34.8	56.9	55.1	427-439 bp	1.0	43.7
ccsA-ndhD_p4	ACAAATTCAGCAATTGTAGCCTT	GTTGAAAAGGTTGAAGTTATCCT	34.8	34.8	57.5	55.1	427-439 bp	1.0	43.7
ccsA-ndhD_p5	ACAAATTCAGCAATTGTAGCCT	TGAAAAGGTTGAAGTTATCCT	36.4	33.3	56.9	52.6	425-437 bp	1.0	43.7