Hotspot

rpl32-trnL-UAG

SSC · 111397-112134 · 738 bp

Back to Glechoma

Pi 0.0061
MarkerSeek score 48.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
738
variable_sites
9
pi
0.0061
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CTGGAAGTGGAACGAAAGGT TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.7 57.9 1456-1481 bp 1.0 78.4
rpl32-trnL-UAG_p2 CTGGAAGTGGAACGAAAGGT AGCAGCGTGTCTACCAATTT 50.0 45.0 57.7 57.8 1449-1474 bp 1.0 77.9
rpl32-trnL-UAG_p3 CTGGAAGTGGAACGAAAGGT CCTAAGAGCAGCGTGTCTAC 50.0 55.0 57.7 57.8 1455-1480 bp 1.0 77.9
rpl32-trnL-UAG_p4 CTGGAAGTGGAACGAAAGGT CTAAGAGCAGCGTGTCTACC 50.0 55.0 57.7 57.8 1454-1479 bp 1.0 77.9
rpl32-trnL-UAG_p5 CTGGAAGTGGAACGAAAGGT GGACTGAAGAGCGGGTACTA 50.0 55.0 57.7 58.2 1996-2021 bp 1.0 77.3