Hotspot

trnF-GAA-ndhJ

LSC · 47606-48239 · 634 bp

Back to Glechoma

Pi 0.00315
MarkerSeek score 61.00
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
634
variable_sites
4
pi
0.00315
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 GGGATAGCTCAGCTGGTAGA GTTGGATAGGATGGCCCTTG 55.0 55.0 58.0 58.0 776-782 bp 1.0 80.3
trnF-GAA-ndhJ_p2 GAGGGTGGGATGCTACATTG GTTGGATAGGATGGCCCTTG 55.0 55.0 58.0 58.0 805-811 bp 1.0 80.1
trnF-GAA-ndhJ_p3 CCTCGTGTCACCAGTTCAAA GTTGGATAGGATGGCCCTTG 50.0 55.0 58.1 58.0 740-746 bp 1.0 79.9
trnF-GAA-ndhJ_p4 GGGATAGCTCAGCTGGTAGA AGTTGGATAGGATGGCCCTT 55.0 50.0 58.0 58.1 777-783 bp 1.0 79.7
trnF-GAA-ndhJ_p5 GAGGGTGGGATGCTACATTG AGTTGGATAGGATGGCCCTT 55.0 50.0 58.0 58.1 806-812 bp 1.0 79.5